Document Type : Original research articles
Authors
1
Department of Nutrition and Food Sciences, Faculty of Home Economics, Menoufia University, Shebin El-Kom, Egypt
2
Department of Nutrition and Food Sciences, Faculty of Home Economics , Menoufia University,Shibin El Kom, Egypt
3
Department of Nutrition and Food Sciences - Faculty of Home Economics - Menoufia University - Egypt
Abstract
This
study investigated the preventive effects of a food supplement with Papaya (Carica papaya) leaves and seeds either alone or in combinations at 2.5 and 5% against Gentamicin-induced Chronic kidney disease (CKD) in rats. Forty-eight rats weighing 150±5g were fed a basal diet for a week adaptation period. Rats were divided randomly into eight groups; each group included 6 rats. The first healthy group fed basal diet only as a Negative (-ve) Control, while the second group fed basal diet as a Positive (+ve) control. Other 6 pretreated groups fed basal diets containing Papaya (Carica papaya) leaves and seeds and their combinations at 2.5 and 5%, respectively, for about 4 weeks. Following the 28-day study period, gentamicin (10 ml/kg body weight) was delivered into all pretreatment groups and the positive control group to cause chronic kidney disease. The rats were weighed weekly during the trial, and their feed consumption and weight increase were noted. Following the experiment's conclusion, rats were sacrificed, blood samples were taken, and their serum was separated, centrifuged, and then frozen at -20°C until analysis. Biochemical analysis such as serum creatinine, urea nitrogen, uric acid, Serum sodium (Na), Serum potassium (K), Albumin, Blood glucose, Serum triglycerides, Total cholesterol, HDL, LDL, VLDL levels, Serum total protein (STP), L-Aspartate aminotransferase (AST), L-Alanine aminotransferase (ALT) and Alkaline phosphatase (ALP) was measured. Additionally, superoxide dismutase (SOD), glutathione reeducates (GSH), and Malondialdehyde (MDA) were measured. The groups treated with higher amounts showed the best results for Papaya leaves and seeds.
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