The Protective Effect of some Vegetables against Oxidative Stress in Mice

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The present study aimed to examine the relation between consumption of freeze-dried tomato and carrot and alterations in kidney, spleen as well as antioxidant enzymes and serum analysis of albino mice. The protective effects of tomato and carrot were assessed against acrylamide that administered to Swiss adult albino mice. The experiment included twenty eight mice. The first group (7 mice) was considered as negative normal. The remaining mice were subjected to daily dose (50 µg acrylamide /kg body weight for 10 weeks. The treated groups were divided into three groups; each seven mice. One of these groups (group 2) was assigned as positive control. Both negative and positive groups were fed on basal diet. The other two groups (Group 3 and group 4) were given diets with formulas with 20% of freeze dried tomato and carrot, respectively. At the end of the experiment, kidney and spleen were histopathologically examined; serum Glutathione (GSH), Glutathione-S-transferase (GST), liver functions, kidney functions and lipid profile were estimated. The diets with vegetables demonstrated significant biochemical protection against acrylamide associated with significant reductions in the activity of (GSH), (GST) serum, liver functions, kidney functions and lipid profile compared to those fed on basal diet (positive control). Histopathological examinations revealed that kidney and spleen tissues were disturbed when mice were fed on acrylamide diet. While mice fed on diets with either tomatoes or carrots exerted protective effects and reversed histopathological changes, all in a dose dependent manner. The obtained results assured that the consumption of tomatoes and carrots ameliorated the harmful effect of acrylamide on both kidney and spleen

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