Comparative Study of Chelated and Non Chelated Iron in biological indices in adult Rats

The main objective of this study is to  compare the absorption configuration and its correlated signs of biological indices of adult male Albino rats by using of different chelated and unchelated iron forms. Thirty two white male albino rats,weighting 145-150g were fed on basal diet for adaptation after adaptation period. Rats were divided into 8 groups (4 rats each group), all groups were fed for 12 weeks on experimental diet as follows: four groups were fed on 50% Fe- deficient diet (17.5mg/kg) as ferrous sulfate, ferric citrate, glycine-chelated iron, lysine-chelated iron. Another four groups were fed on adequate Fe diet (35mg/kg) as ferrous sulfate, ferric citrate, glycine-chelated iron, lysine-chelated iron. At the end of the experimental period  (12 weeks), rats were fasted over night before sacrificing, blood were collected to determine biological analysis. The obtained  results  revealed that groups of rats fed on iron amino acid chelate were significantly increased than ferrous sulfate on hemoglobin. The results were clearly that Red blood cells and HCT was the highest in groups feeding on Fe-diets as glycine type comparing to other groups under study. Red blood cell distribution width, it could be noticed that RDW% of group of rats fed on adequate Fe diets as glycine-chelated iron showed the lowest level and significantly decreased when compared to groups of  rats fed on  50% Fe- deficient diets as ferrous sulfate, ferric citrate, glycine-chelated iron and lysine-chelated iron.Serum ferritin, the best result was recorded for the group of rats fed on adequate Fe as glycine chelated iron which it. Serum iron the best results were recorded for groups of rats fed on adequate Fe diet as ferric citrate, glycine -chelated iron. Results concluded that iron amino acid chelate had a positive impact on hematological indices and iron status of adult rats.