Nutraceutical Effect of Diets Containing Graviola Fruit Parts on Hepatointoxicated Rats.

This study aimed to investigate the effect of different Graviola (Annona muricata, L.) fruit parts including (Pulp), (Green Peel), (Black Seeds) and their blend on amelioration the hepatotoxicity in CCl₄ injected rats. Fifty (50) adult male albino rats, weighing (180±10g) were divided into ten groups, five rats each. Group 1 was fed on the basal diet and set as a negative control group (normal rats), meanwhile the nine other groups were injected by 0.2 mg/kg body weight by Carbon Tetrachloride (CCl₄) for two weeks to induce the liver impaired. Group 2 was left as a positive control group and fed on basal diet only, while other groups 3, 4, 5, 6, 7, 8, 9 and 10 were fed on supplemented diet with 5% and 7.5% powder of Annona pulp, Annona peels, Annona seeds and mixture of all, respectively. Body weight, feed intake, feed efficiency ratio, internal organs weight, serum glucose, liver functions, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) enzymes, total protein, total cholesterol, triglycerides, high-density lipoproteins cholesterol (HDLc), low-density lipoproteins cholesterol (LDLc) and very low-density lipoproteins cholesterol (VLDLc), kidney functions (urea, creatinine and uric acid), total bilirubin, direct bilirubin, indirect bilirubin, antioxidant enzymes (Catalase, Superoxide Dismutase, and Glutathione Peroxidase) assayed. Histopathological changes of liver and kidney have been evaluated. From the obtained results, it was revealed that feeding on Graviola fruit parts powders (G3, G4, G5, G6, G7, G8, G9 & G10) caused a significant (P ≤ 0.05) increase in weight gain, feed intake, feed efficiency ratio, HDLc, total protein, albumin, globulin, CAT, SOD, GPx with a significant (P ≤ 0.05) decreases in the rest of the analyses as compared with control (+ve) group, and enhanced the kidney and liver functions with the decrease of ALT, AST, ALP, total bilirubin, indirect bilirubin, albumin, globulin, which reflects the powerful nutraceutical therapeutic effect for feeding on Graviola fruit parts including (The Pulp), (Green Peel), (Black Seeds) and their blend to combat the hepatointoxication induced by CCl₄ in male albino rats. The best treatments were for Annona pulp 7.5%.