Fighting Hepatotoxication with CCl4 of Male Albino Rats using Plant Flowers

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This study amied to investigate the effect of white frangipani (Plumeria  alba) ,chamomile (Matricaria chamomilla or chamomile  recutita capitula),clove ( Syzygium aromaticum ) ,safflower(Carthmus tinctorius)and their blend on amelioration the hepatotoxicity in Carbon Tetrachloride CCl4  injected rats .Sixty (60) adult male albino rats, weighing (150±10 g) were divided into (12) groups, each with five rats. One of them was kept as a control (-ve) Group, while the rats of  the other eleven groups were injected by   0.2 mg/kg body weight by Carbon Tetrachloride for two weeks (twice weekly) to induce the liver impaired.  The flower powders were added at percent  of 2 % and 4% from the basal diet and given as single and as a mixture.Glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and alkaline phosphatase (ALP), total bilirubin, direct bilirubin, indirect bilirubin, albumin, total protein , lipid profile : cholesterol(TC), tri-glycerides (TG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), very low density lipoprotein cholesterol (VLDL-c), atherogenic index (AI), urea, creatinine & uric acid assayed and histopatholigical changes were examined. Phenolic compounds and phenolic analysis of flowers were determined .The results indicated that rats treated with  CCl4  recorded significant changes  for all above biological  and biochemical parameters. Histopathological examination supported  these findings and revealed amarked lesion in the inflicted  rats . HPLC analysis of safflower,clove,chamomile and white frangipani flowers has revealed  the increase contents of these flowers of Phenolic compounds.  From the obtained resultsthe mixture  of all flower powders    enhanced  the  liver functions, lowering GOT and GPT enzymes. As regards to serum total bilirubin, indirect bilirubin, albumin, globulin,   the best treatments are considered that of themixture  of all flower powders  4%. Histopathological investigation confirmed the biochemical changes considering the liver and kidney functions.

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